Use 0.5 mg Histrelin when follicles are ≥ 35 mm to advance ovulation.
PROTOCOL FOR USING P4 150 FOR MANAGING ANOVULATORY OR OVARIECTOMIZED RECIPIENTS
When the donor is day 2 or 3 of estrus, give recipient 5 to 10 mg Estradiol Cypionate for 2 to 3 consecutive days
No other injections until the donor is day 2 post ovulation
When the donor is day 2 post ovulation, give the recipient 5 mL of P4 300, IM
On the day of embryo transfer, give the recipient P4 300, IM, and every 7 days thereafter until day 110 of pregnancy
If desirable group all recipients such that all injections of P4 300 are given on the same day of the week. There is no contraindication for giving the P4 300 at less than 7 day intervals. For example, the injections could be given on Monday and again on Thursday to get all recipients on the same schedule